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KMID : 1007520030120040415
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Food Science and Biotechnology
2003 Volume.12 No. 4 p.415 ~ p.418
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Quantitative Analysis of Two Major Flavonoid Aglycones in Acid Hydrolyzed Samples of Angelica keiskei by HPLC
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Lee, Jeong-Hwan
Lee, Ja-Young/Kim, Kyung-Nam/Kim, Hyun-Su
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Abstract
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The acid hydrolysis method was applied for analyzing flavonoid aglycones in Angelica keiskei. The experiment examined the leaves and stalks of the plant separately. HPLC (High Performance Liquid Chromatography) with diode array detection was used to identify of five major flavonoids； quercetin, kaempferol, myricetin, luteolin and apigenin. Quercetin and luteolin were found in the leaves, and only quercetin was found in the stalk. The hydrolysis conditions of the two flavonoid aglycones in the leaves were optimized by varying acid concentrations and reaction periods. The range of each parent flavonoid content was determined by optimized hydrolysis conditions. The samples were harvested from two areas of Korea between February and August 2002. Quercetin content was 90㎎ at the lowest and 809㎎ at the highest per ㎏ of fresh leaves. The content of luteolin ranged between 82㎎ and 579㎎ per ㎏ of fresh leaves. The quercetin content in the stalk was much lower than in the leaves, and luteolin was not detected in the stalk. The correlation between flavonoid content and sunshine durations was also presented.
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